Investigation of Antimicrobial Activity and DNA Protective Capacity of Melissa officinalis Extracts

Abstract

In this study, antimicrobial activity, antioxidant capacity of Melissa officinalis leaf extracts and DNAprotective effect aginst H 2 O 2 -induced oxidative damage were investigated. DNA protective effect ofMelissa officinalis's leaf extract from oxidative stress was determined by using yeast comet assay. Thecomet assay, applied on Saccharomyces cerevisiae BY4741. We observed that DNA damage decreasedin a dose dependent manner in experiments of preincubation and simultaneous incubation with theextract upon oxidative shock. Results indicated that Melissa officinalis's methanolic leaf extract protectthe DNA against the damaging effect of hydrogen peroxide. Also we indicated that the antimicrobialeffects of Melissa officinalis's extract on the different microorganisms. Extract of Melissa officinalisshowed antimicrobial effect on all test test microorganisms.