Investigation of single strand DNA breaks in spontaneous abortion cases with known pesticide exposure

Abstract

Pesticide exposure is an essential public health problem and endocrine-disrupting factor. Encounters during pregnancy may cause spontaneous abortions or congenital anomalies with embryotoxic or fetotoxic effects. DNA double-strand breaks occur upon exposure of DNA to radiation and chemicals or are caused by faulty DNA metabolic processes. This research examines the relationship of DNA single-strand amount with pesticides and their types. Single-stranded DNA (ssDNA) density was quantitatively determined by fluorometric method in the blood of Spontaneous Abortion with organochlorine and or organophosphate pesticide exposure and in the blood of two patient groups who had normal delivery without pesticide contact. The mutation was screened by next-generation sequencing in 4 women who experienced spontaneous abortion with a high pesticide amount and variety. 91 female patients aged 18-35 years were included in the study. The spontaneous abortion group was 56 (61.5%), and the normal delivery group was 35 (38%) women. The mean age was 28.18(±4.5). There was a significant correlation between Pentachlorophenol -52 (PCB52) and ssDNA level (p<0.05). There was no relationship between ssDNA ratios and groups (p>0.05). It was determined that ssDNA levels were higher among those with multiple abortions. The ssDNA level was higher in those who had an abortion before (p<0.05). We determined that dichloro di phenyl tri chloro ethane (DDT) and its congeners were significantly higher in the Whole Exome Sequence analysis of 2 of the four patients with the highest pesticide level. The DYNC2H1 gene NM_001377.3 c.7506G>A (p.Trp2502Ter) variation and the DYNC2H1 gene NM_001377.3 c.8458C>A (p.Pro2820Thr) variation associated with recurrent miscarriages were found to be heterozygous. Studies in this area need to be increased to determine the relationship of ssDNA elevation with pesticide exposure, its predictive value in abortion, and the value of environmental factors in repeated abortions whose etiology has not been clarified.