Suppressive effects of resveratrol on epithelial-mesenchymal transition in the YKG1 glioblastoma cell-line

Abstract

Glioblastoma multiforme (GBM), is an aggressive tumor type with high mortality and morbidity, which a curative treatment method has not been developed yet. Although Epithelial-mesenchymal transition (EMT) is a known mechanism to be effective in the cancer process, the EMT process seen in these cells has not been fully explained yet. Resveratrol is a flavonoid and has been reported to have positive effects on different types of cancer. In this study, we aimed to compare the effects of resveratrol on EMT in YKG1 GBM cell line. Different doses of resveratrol (50 and 100 ?M) were applied to YKG1 glioblastoma cells, and their viability was calculated and immunocytochemical analyzes were evaluated. According to results, it was observed that the 100 ?M resveratrol dose significantly reduced the viability compared to the 50 ?M resveratrol dose. It was determined that Vimentin and N-Cadherin H scores of 100 ?M Resveratrol dose decreased and E-cadherin and Claudin-1 values increased compared to the other groups. The results showed that increasing resveratrol dose was more effective on EMT on glioblastoma cell lines in a dose-dependent manner.