Evaluation of IKK-?, NF-k?, p53, and Ki-67 Protein and Gene Expression in Neuroblastoma Cells Treated with Cisplatin

Abstract

Background: Neuroblastomas are extracranial solid tumors caused by the differentiation and uncontrolled proliferation of immature sympathetic nervous system cells. This study investigated the effects of the anticarcinogenic cisplatin (Cis) on I??-?, NF-??, p53, Ki-67 protein, and gene expression in neuroblastoma cells. Materials and Methods: SH-SY5Y cells were treated for 48 hours with various doses of Cis (1, 3 and 10 ?M). IKBK1, MKI67, TP53, and NFKB1 gene expression analyses were completed by transcription-quantitative polymerase chain reaction (RT-qPCR). The levels of I??-?, NF-??, p53, and Ki-67 proteins were detected by Western blotting. Results: IKBKB gene expression was significantly increased at 1 and 3 µM and reduced significantly 10 µM compared to the control. NFKB1 gene expression significantly increased at 1 ?M Cis, whereas it decreased when Cis concentration increased. TP53 gene expression significantly increased at 1 and 3 µM. MKI67 gene expression decreased with an increase in Cis concentration. In the low concentration (1 µM) of Cis group, while there was no significant change in I??-? and p53 protein expressions, NF-?? expression significantly increased. However, in the groups administered with high-dose Cis (3 µM, 10 µM), where Cis showed a significant antiproliferative effect, I??-? expressions decreased significantly, while NF-?? and p53 expression increased. The expression of the proliferation marker Ki-67 was also reduced in this group. Conclusion: These results highlight the antiproliferative activity of Cis and its related NF-??/IKK-?, p53, and Ki-67 proteins and the marked changes in gene expression in neuroblastoma cells.