Protective Feature of Anzer Propolis in Contrast-Induced Nephropathy in Rats

Abstract

Aim: The study aims to ascertain whether Anzer Propolis, a natural antioxidant compound, can protect the kidneys from iopromide. Material and Method: This study was done in 2021. Four groups of 32 adult male Wistar albino rats, weighing between 250 and 300 g, were formed. Control (C) group contained eight rats. Intraperitoneal ethanol was administered to the Ethanol (E) group (n=8), 8 g/ kg intraperitoneal iopromide to the contrast-induced nephropathy (CIN) group (n=8), and a 100 mg/kg dose of Anzer Propolis on the date of application to the Anzer Propolis group (AP) (n=8), as well as the prior day, by gastric gavage and then 8 g/kg iopromide was administered intraperitoneally. Termination was carried out at the 48th hour. Histopathological examination was performed for CIN in the right and left kidneys. Results: Following has been examined as a part of the research: serum urea, creatinine, IL6, interleukin (IL)-1?, total oxidant status (TOS), tumor necrosis factor (TNF-?), total antioxidant status (TAS), myeloperoxidase (MPO). TAS had a statistically significant increase in AP, in comparison to CIN, C and E groups (p<0.05). The pathological examination showed, vacuolar degeneration formations in tubular epithelial cells, and increase in mononuclear cell infiltration areas in the interstitial region in CIN. CIN had significantly higher values in comparison to AP (p<0.05). Conclusion: Propolis protects the kidney tissue against the toxicity caused by CIN and negative effects of free radicals; most likely due to the antioxidant and antitoxic properties. Propolis is a promising natural substance for preventing CIN. There is a need for further research on the properties of propolis.