ANALYSIS OF POLYMORPHIC CHROMOSOME VARIANTS IN COUPLES WITH RECURRENT PREGNANCY LOSS

Abstract

Objective: This study evaluated chromosome polymorphisms (1qh+, 9qh+, inv9, 13ps+, 14ps+, 15ps+, 16qh+, 21 ps+, 22 ps+ and Yqh+) in a case group (n=1688) with two or more recurrent pregnancy losses (RPL) and a control group (n=80). Materials and Methods: The control group was selected from 40 married couples who had no known hereditary disease, were not relatives, had healthy children, and had no history of miscarriage and/or stillbirth. Phytohemagglutinin-induced peripheral blood lymphocytes were cultured for 72 h. The Giemsa–Trypsin–Leischman (GTL) banding technique was applied to the obtained metaphase plates; thirty metaphase plates were examined at the 450-550 band level in each individual. Results: A total of 488 individuals in the case group and 13 in the control group carried polymorphic chromosome variants.9qh+ chromosome polymorphisms were more prevalent in the case group than in the control group (p=0.028). Other variants were also increasingly observed in the case group (p=0.014). Conclusion: Our findings reveal a potential relationship between RPL and chromosome polymorphisms. Karyotype analysis and appropriate genetic counseling increase the chance of having healthy children in individuals with RPL.