| dc.description.abstract | Hypoxia, during early embryonic development, causes morphological and functional impairments through oxidative stress. Melatonin, a strong antioxidant, may exert potential protective effects in this process. This study investigated the effects of melatonin on yolk sac vascularization and embryonic development in rat embryos exposed to hypoxia. Rat embryos at gestational day 9.5 were cultured for 48 h using a whole embryo culture (WEC) system. Embryos were divided into six groups: Control (C), Hypoxia (H), 10 ?M Melatonin (10 ?M Mel), Hypoxia +10 ?M Melatonin (H+10 ?M Mel), 50 ?M Melatonin (50 ?M Mel), and Hypoxia +50 ?M Melatonin (H+50 ?M Mel). At the end of culture, morphological parameters, oxidative stress markers, and the distribution of vWF-positive cells were evaluated. Compared with the control group, the H group exhibited marked developmental delay and vascularization defects (P<0.05). Melatonin administration significantly reduced these abnormalities, with the 50 ?M melatonin group showing the most prominent improvements in morphological development, oxidative stress parameters, and vascularization (P<0.05). Melatonin provides protective effects against hypoxia-induced early embryonic developmental impairments and holds therapeutic potential as an agent supporting development during the early intrauterine period. © The Author(s) 2025. Published by Oxford University Press. All rights reserved. | |
| dc.department-temp | Nisari, Mehtap, Department of Anatomy, Erciyes University, Faculty of Medicine, Kayseri, Turkey; Patat, Dilara, Department of Anatomy, Ankara Medipol University, Ankara, Turkey; Sengül, Göksemin Fatma, Department of Medical Biochemistry, Ankara Medipol University, Ankara, Turkey; Ü?ger, Harun, Department of Anatomy, Erciyes University, Faculty of Medicine, Kayseri, Turkey; Ertekin, Tolga, Department of Anatomy, Afyonkarahisar Health Sciences University, Afyonkarahisar, Afyonkarahisar, Turkey; Yay, Arzu Hanım, Department of Histology and Embryology, Erciyes University, Faculty of Medicine, Kayseri, Turkey; Şentürk, Meryem, Veterinary Biochemistry, Erciyes Üniversitesi, Kayseri, Kayseri, Turkey; Çayan, Dicle, Faculty of Science and Arts, Nigde, Turkey; Ömerli, Ayşe, Department of Anatomy, T. C. Nuh Naci Yazgan Üniversitesi, Kayseri, Turkey; Al, Özge, Department of Anatomy, Erciyes University, Faculty of Medicine, Kayseri, Turkey; Uçar, Sümeyye, Department of Anatomy, Erciyes University, Faculty of Medicine, Kayseri, Turkey; Güler, Hatice Susar, Department of Anatomy, Erciyes University, Faculty of Medicine, Kayseri, Turkey; Göçmen, Ayşe Yeşim, Department of Medical Biochemistry, Bozok Üniversitesi, Yozgat, Turkey; Yilmaz, Seher, Dep | |
