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dc.contributor.authorSarıkürkçü, Cengiz
dc.contributor.authorŞahinler, Saliha Şeyma
dc.contributor.authorCeylan, Olcay
dc.contributor.authorTepe, Bektaş
dc.date.accessioned2021-05-05T22:14:06Z
dc.date.available2021-05-05T22:14:06Z
dc.date.issued2020
dc.identifier.issn0926-6690
dc.identifier.issn1872-633X
dc.identifier.urihttps://doi.org/10.1016/j.indcrop.2020.112651
dc.identifier.urihttps://hdl.handle.net/20.500.12933/285
dc.descriptionSarikurkcu, Cengiz/0000-0001-5094-2520; ceylan, olcay/0000-0002-4435-8405en_US
dc.descriptionWOS:000554526900038en_US
dc.description.abstractDue to their medical importance, the number of studies on the enlightenment of the biological activities of Onosma species has been increasing over the years. In this study, besides the phytochemical composition of Onosma ambigens Lacaita (Boraginaceae), in vitro antioxidant, tyrosinase and alpha-amylase inhibitory activities of extracts obtained by using different solvents (ethyl acetate, methanol and water) were investigated. As a result of the spectrophotometric analysis, it was determined that methanol extract was rich in both phenolics (51.19 mg GAEs/g extract) and flavonoids (45.39 mg QEs/g extract). According to the results of liquid chromatography electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) analysis, the major components of methanol extract were determined as rosmarinic acid (37670.55 mu g/g), luteolin 7-glucoside (15756.40 mu g/g), apigenin 7-glucoside (15080.34 mu g/g), luteolin (2289.77 mu g/g), apigenin (2240.38 mu g/g), chlorogenic acid (2121.92 mu g/g), vanillic acid (1179.66 mu g/g), hesperidin (1038.38 mu g/g), 4-hydroxybenzoic acid (961.97 mu g/g) and pinoresinol (631.61 mu g/g). It was found that methanol extract showed remarkable activity in almost all antioxidant activity tests (except ferrous ion chelating test). The activity of this extract in the phosphomolybdenum, reducing power [cupric reducing antioxidant capacity (CUPRAC) and ferric reducing antioxidant power (FRAP)] and radical scavenging [on 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS)] assays were measured as 1.65, 0.95, 0.52, 1.86 and 1.45 mg/mL, respectively. Phenolic and flavonoid contents, especially chlorogenic acid, vanillic acid, luteolin 7-glucoside, hesperidin and rosmarinic acid, are thought to contribute significantly to the antioxidant activity of the extracts. In tyrosinase and a-amylase inhibitory activity tests, methanol (2.81 mg/mL) and ethyl acetate (2.64 mg/mL) extracts exhibited the best activity among the others, respectively. While it is thought that chlorogenic acid and rosmarinic acid may contribute to the tyrosinase inhibitory activity of methanol extract, it has been concluded that detailed chromatographic analyzes should be performed for detection of the compounds responsible for alpha-amylase inhibitory activity.en_US
dc.language.isoengen_US
dc.publisherElsevieren_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectOnosma ambigensen_US
dc.subjectAntioxidant activityen_US
dc.subjectalpha-Amylaseen_US
dc.subjectTyrosinaseen_US
dc.subjectPhytochemistryen_US
dc.subjectLC-ESI-MS/MSen_US
dc.titleOnosma ambigens: Phytochemical composition, antioxidant and enzyme inhibitory activityen_US
dc.typearticleen_US
dc.departmentAFSÜ, Eczacılık Fakültesi, Temel Eczacılık Bilimleri Bölümüen_US
dc.contributor.institutionauthorSarıkürkçü, Cengiz
dc.contributor.institutionauthorŞahinler, Saliha Şeyma
dc.identifier.doi10.1016/j.indcrop.2020.112651
dc.identifier.volume154en_US
dc.relation.journalIndustrial Crops And Productsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US


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