The combination of circulating levels of ANGPTL, omentin-1, leptin and cytokines is associated with polycystic ovary syndrome in different BMI groups

Abstract

Objective: This study aims to explore the combined role of ANGPTL3, 4, 8, omentin-1, leptin, TNF-alpha, and IL-6 molecules known for their roles in fat metabolism, obesity, and inflammation, yet whose connection to PCOS is still debated in the development of PCOS. Methods: A prospective cross-sectional study involving PCOS patients [n=30] and BMI-matched controls [n=30] was conducted. Levels of ANGPTL3, 4, 8, omentin-1, leptin, TNF-alpha, and IL-6 were measured in peripheral venous blood samples. Results: When dividing both the PCOS and control groups into six BMI-based subgroups normal weight [20-24.9 kg/m(2)], slightly overweight [25-29.9 kg/m(2)], and obese [30-39.9 kg/m(2)] there were significant differences in levels of ANGPTL3 and 8, omentin-1, leptin, IL-6, and TNF-alpha [p<0.05]. Comparison between the entire PCOS and control groups showed that CRP levels were significantly higher in the PCOS group [p<0.0001], while omentin-1 levels were significantly lower [p=0.022]. Regression analysis, including ANGPTL3, 4, 8, IL-6, andTNF-alpha alongside CRP and omentin-1, indicated a significant model for PCOS [Nagelkerke R-2=0.698, p<0.0001, PPV=80%, NPV=90%]. In ROC analysis, the AUC for CRP and omentin-1 were significant [p<0.05; AUC=0.800-0.328]. Conclusion: This study suggests a continuous interaction among ANGPTL, omentin-1, leptin, and cytokines in the etiopathogenesis of PCOS.