The importance of CYP1B1 polymorphism in obesity

Abstract

Obesity is a multifactorial disease, commonly observed both worldwide and in our country, triggered by environmental and genetic factors, adversely affecting all physiological functions of the body, and leading to an increase in body fat mass. Although various variants associated with susceptibility to obesity have been identified in genomic studies, these variants explain only a small portion of the genetic basis of obesity. This case-control study investigates, for the first time in the Turkish population, the relationship between CYP1B1 gene rs1056827 and rs1056836 polymorphisms in obesity patients undergoing surgical intervention (bariatric surgery). Genotyping of the polymorphisms was performed using Real-Time PCR in 63 female and 29 male obesity patients who underwent bariatric surgery and 40 female and 51 male nonobese individuals. In our study, genotype distributions for the CYP1B1 gene rs1056836 polymorphism were found to be 51.1% CC, 40.2% CG, and 8.7% GG in the case group and 46.2% CC, 47.3% CG, and 6.6% GG in the control group. The frequency of the C allele was 71.2%, and the G allele was 28.8% in the case group, while the frequency of the C allele was 70.3%, and the G allele was 29.7% in the control group. For the rs1056827 polymorphism, the genotype distributions were 10.8% GG, 35.9% GT, and 53.3% TT in the case group and 7.7% GG, 49.4% GT, and 42.9% TT in the control group. The frequency of the G allele was 28.8%, and the T allele was 71.2% in the case group, whereas the frequency of the G allele was 32.4%, and the T allele was 67.6% in the control group. No significant difference was found between the case and control groups in terms of anthropometric measurements and biochemical parameter values for the rs1056836 and rs1056827 polymorphisms of the CYP1B1 gene. Our study is valuable as it is the first to investigate the association of CYP1B1*2 (rs1056827) and CYP1B1*3 (rs1056836) polymorphisms with obesity, and it was determined that there was no difference in the investigated polymorphisms between the control group and the obesity group.