SIMULTANEOUS SPECTROPHOTOMETRIC QUANTITATION OF RUTIN AND CHLOROGENIC ACID IN LEAVES OF Ribes uva-crispa L. BY ONE-DIMENSIONAL CONTINUOUS WAVELET TRANSFORMS

Abstract

New signal processing methods based on one-dimensional continuous wavelet transforms were proposed for the simultaneous quantification of chlorogenic acid and rutin in the leaves of Ribes uva-crispa L. These methods are based on the application of continuous wavelet transforms to the absorption spectral bands of the analyzed compounds and their samples. In preliminary practices, symlets 8 and biorthogonal 4.4 continuous wavelet transforms (sym8-CWT and bior4.4-CWT) among several wavelet families were found to be optimal ones to perform a selective and sensitive chemical determination of the analytes in analyzed plant samples. Linear regression lines were obtained by measuring sym8-CWT and bior4.4-CWT amplitudes at 333.5 and 336.0 nm for chlorogenic acid and 351.5 and 350.0 nm for rutin in the spectral region 250-450 nm. Linear regression curves were found to be linear in the working range of 2.5-40.0 mu g/mL for both compounds. The validation and analytical applicability of the proposed sym8-CWT and bior4.4-CWT approaches were confirmed by analyzing the synthetics mixtures and standard addition samples. It was observed that the application of both sym8-CWT and bior4.4-CWT tools is very appropriate for the simultaneous spectral quantification of chlorogenic acid and rutin in the real plant samples consisting of leaves of R. uva-crispa.