LC–ESI–MS/MS characterization of phytochemical and enzyme inhibitory effects of different solvent extract of Symphytum anatolicum

Abstract

Symphytum anatolicum Boiss., is a species belonging to the Boraginaceae family and have been used for its curative effects. This work investigated, for the first time, the bioactive compounds, the antioxidant activities and the enzyme inhibitory effects of Symphytum anatolicum roots extracts using three solvents (methanol, water and ethyl acetate). The LC–ESI–MS/MS was used to identify the individual bioactive compounds. Twenty-three biomolecules were identified in the extracts and rosmarinic acid, verbascoside, caffeic acid, chlorogenic acid and vanillin were the main identified with varied levels depending on the solvent used. Using six different assays, all extracts exhibited important antioxidant activities. Results showed also that the methanol extract exerted better tyrosinase inhibitory activities while ethyl acetate extract showed the best ?-amylase inhibition. The obtained results highlighted the importance of S. anatolicum as a natural resource of bioactive compounds and encourage the use of this unexploited species for food additives, pharmaceutical industries, and skincare products. © 2019 Elsevier B.V.