Investigation of the effects of boron on a renal ischemia/reperfusion injury in rats
AuthorSarıtaş, Tuba Berra
Bozkurt, Mehmet Fatih
Sarıtaş, Zülfükar Kadir
MetadataShow full item record
Purpose: This study was intended to investigate the effects of boron on a rat renal ischemia/reperfusion (I/R) damage model on biochemical and histopathological grounds. Methods: The control, sham, I/R, and Boron groups in the study included a total of 24 female Wistar rats with weights of 250-350 grams. Groups I/R and B underwent laparotomy under the general anesthesia and the researchers dissected left kidney pedicles of all experimental animals. A kidney artery clamp was applied for 1 hour to generate ischemia, which was followed by 6 hours of reperfusion. Group C also received 10 mg/kg boron intra peritoneally after I/R. Once blood samples were taken from the rats, they were sacrificed and tissue samples were taken by means of nephrectomy. The study analyzed the serum samples seeking Myeloperoxidase (MPO), Ischemic Modified Albumin (IMA), Malondialdehyde (MDA), Nitric Oxide (NO), Superoxide Dismutase (SOD), Glutathione Peroxidase (GPx), Antioxidant Activity (AOS), Urea, and Creatinine. The researchers also measured MPO, MDA, NO, SOD, GPx, and AOS in the tissue samples. Results: The IMA level in the serum samples was significantly lower in group B than group I/R (P<0.05). The MDA level in the serum samples was significantly higher in group I/R than in groups C and S (P<0.05). The AOS level in the serum samples was significantly lower in groups B and I/R than in groups C and S (P<0.05). The histopathologic scoring was significantly lower in group B than in group I/R (P<0.05). Conclusion: Boron in the renal I/R injury model makes a protective effect against I/R damage by decreasing oxidative stress in serum and tissue samples. This result was supported by histopathological examination.