Advanced Search

Show simple item record

dc.contributor.authorSabaner, Mehmet Cem
dc.contributor.authorDuman, Reşat
dc.contributor.authorVurmaz, Ayhan
dc.contributor.authorErtekin, Tolga
dc.descriptionSabaner, Mehmet Cem/0000-0002-0958-9961; VURMAZ, AYHAN/0000-0002-1840-2900en_US
dc.descriptionPubMed: 33461354en_US
dc.description.abstractBackground: To investigate the effects of bimatoprost, latanoprost and travoprost on angiogenesis in the chick chorioallantoic membrane (CAM) model in ovo Materials and methods: Fifty fertilized specific-pathogen-free chick eggs were used in this preclinical, prospective, experimental embryo study. Eggs were randomly distributed into 5 groups of ten eggs. Eggs were placed in the incubator after disinfection of their shells with alcohol and monitored appropriate temperature and humidity. On the 3rd day of incubation, a small window was opened on the eggshell. Bimatoprost in group 1, latanoprost in group 2, travoprost in group 3, bevacizumab in group 4, phosphate-buffered-saline (PBS) used in group 5 was applied by injection to CAM. The sterile film was glued onto the broken part of the shell and the eggs were placed in the incubator again. On the 8th day of incubation, eggs were opened and vascular structures on CAMs were examined. Digital photographs were taken, analysed in the ImageJ open source image processing software and differences between groups were evaluated. Thereafter, VEGF (Vascular endothelial growth factor) levels were measured appropriately in the embryo samples. Results: All embryos in the prostaglandin groups and the PBS control group were observed to have life signs confirmed by heart rate. In 8 embryos in the bevacizumab group, no life signs were confirmed, while 2 embryos with life signs showed severe hypoplasia. Vascular density, number of vessels and VEGF levels in the bimatoprost, latanoprost and travoprost groups, there were statistically significantly higher than the PBS control group. Conclusion: This study demonstrates that topical prostaglandin drops increase angiogenesis in the chick CAM model in ovo.en_US
dc.publisherTaylor & Francis Ltden_US
dc.subjectchorioallantoic membraneen_US
dc.titleEffects of topical prostaglandin drops on angiogenesis in an in ovo chick chorioallantoic membrane modelen_US
dc.departmentAFSÜ, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Tıbbi Biyokimya Ana Bilim Dalıen_US
dc.contributor.institutionauthorVurmaz, Ayhan
dc.contributor.institutionauthorErtekin, Tolga
dc.relation.journalCutaneous And Ocular Toxicologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US

Files in this item


This item appears in the following Collection(s)

Show simple item record